RNA dot blot protocol
RNA dot blot protocol
Slots have a small surface area for a high-intensity signal — generates faster results · Small slot size requires less sample than dot-blot systems · Accepts
Assemble the dot blot apparatus with a pre-wetted nylon membrane Load denatured viral DNA and linearized standard plasmid DNA solutions into the wells This apparatus is intended for indoor use only Our Blotting units provide a mechanism for performing “dot-blot” and “slot-blot” DNA:DNA and DNA:RNA
starwin88 slot DotSlot Blotting · Slow or No Filtration of the Sample Through the Membrane · Little or No Protein Observed on the Blot · Stained Blot Is Not Uniform gDNA serial dilutions and the RNase treated gDNA were spotted on a positively charged Hybond-N+ nylon membrane using a dot-blot apparatus The
